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studied 825 healthy European adults at low to below average 10-year cardiovascular risk and free of metabolic syndrome and liver disease and a control group of 154 apparently healthy non-obese subjects at low to below average CV risk and free of metabolic syndrome and liver disease [49]

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Direct Infusion Mass Spectrometry (shotgun lipidomics) offers high-throughput capabilities by introducing lipid extracts directly into the mass analyzer, though it requires exceptional mass resolution and/or tandem mass spectrometry (MS/MS) to differentiate similar lipid species effectively (Ryan and Reid, 2016

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